Fig. 5. Expression of Hub does not affect the release of proinsulin/insulin. Cells were infected with adenovirus, sorted and labelled as described in the legend to Fig. 3. Samples of medium, following a 150 minute incubation under basal conditions and a subsequent 60 minute period of stimulation, as well as cell extracts (after the stimulation) were analysed by HPLC (see Fig. 2). Labelled proinsulin and insulin (corrected for the difference in the number of leucines in each) is presented as a percentage of that found in all samples (basal + stimulated medium + extracts). Data are mean±s.e.m., n=4.