Fig. 2. Runx2 resides in distinct subnuclear domains. HeLa cells transiently expressing Runx2 were processed for NM-IF preparation and double-label immunofluorescence to assess to what extent it colocalizes with nucleolin (A), coilin (B), SC35 (C) and PML bodies (D). Arrowheads point to distinct coilin and Runx2 domains (B) and PML bodies and Runx2 (D). Bars (2 µm) represent the relative magnification of the images.