Fig. 5. ZW10 and ROD co-fractionate from a sizing column. (Top) Fractions of a Drosophila embryo extract were eluted from a Superose 6 sizing column, transferred to a western blot, and probed with antibodies against DmROD. (Bottom) The same fractions probed with DmZW10 antibodies on a separate blot. Lanes at the far right show DmROD and DmZW10 in the material loaded onto the column. 0.5 ml fractions were collected; the void was at fraction 13, and the salt front in fraction 42. Positions of standards are marked below the blot; 669 kDa (thyroglobulin), 66 kDa (bovine serum albumin), 158 kDa (aldolase).