Fig. 1. Intracellular localization of wild-type syntaxins. Clone 9 cells were transfected with HA-tagged wild-type syntaxins 5, 6, 7 and 8 as described in Materials and Methods. After incubation for 12 hours, the cells were fixed and stained with anti-HA antibody and antibodies against indicated marker proteins. Syntaxins 5 and 6 overlapped with ß-COP and TGN38, respectively. Syntaxin 7 was colocalized with EEA1 but not with lamp-1, and syntaxin 8 was partially colocalized with both EEA1 and lamp-1.