Fig. 3. The cytoplasmic domains are important for the intracellular localization of syntaxins. Clone 9 cells were transfected with HA-tagged chimeras composed of the transmembrane domain of syntaxin 1A and the cytoplasmic domain of either syntaxin 5, 6, 7 or 8. After incubation for 12 hours, the cells were fixed and stained with anti-HA antibody and antibodies against indicated marker proteins. Each chimera localized to the same compartment(s) as did its wild-type counterpart with the same cytoplasmic domain.