Fig. 4. Immunofluorescence analysis of chimeric syntaxins in BFA-treated cells. Clone 9 cells were transfected with HA-tagged syntaxin 5 (a,a'), syn5-1 (b,b'), syn6-5 (d,d'), syntaxin 6 (e,e'), and syn6-1 (f,f') and PC12h cells were transfected with HA-tagged syn1-5 (c,c') in the absence (a-f) or presence (a'-f') of BFA. After incubation for expression, the cells were treated with 5 µg/ml BFA for 30 minutes before fixing. The cells were stained with anti-HA antibody. In BFA-treated cells, chimeras containing either the cytoplasmic domain or the transmembrane domain of syntaxin 5 were redistributed from the CGN into the ER, as was syntaxin 5 (a'-d'). By contrast, in BFA-treated cells, syntaxin 6 and syn6-1 were redistributed from the TGN to the MTOC (e',f').