Fig. 1. Localization of AnxII and GFP-AnxII fusion proteins in U1242MG cells. Endogenous AnxII was visualized by indirect immunofluorescence and imaged using epifluorescence photomicrography (AnxII IF). Stably transfected cells expressing EGFP, EGFP-AnxII, EGFP-AnxII(1-22), EGFP-AnxII(23-338) or EGFP-AnxII(L10A/L12A) were fixed and imaged using LSCM. The association of EGFP-AnxII(23-338) with nucleoli is an artifact of fixation, apparently due to residual Ca²⁺ from the wash buffer. The construct is excluded from nucleoli in living cells (not shown). Bar, 20 µm.