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Fig. 4. Immunolocalization of {alpha}T-catenin in cryosections of human testis. (A) Double immunofluorescent staining of {alpha}T-catenin (monoclonal antibody 892_24D2S) and {alpha}E-catenin (polyclonal antibody) shows differential localization of these two related proteins. The {alpha}T-catenin is present in peritubular cells, clearly separated from {alpha}E-catenin, which is present in cells within the seminiferous tubules. (B) Immunohistochemical staining of consecutive sections for {alpha}T-catenin (monoclonal antibody 892_24D2S) and desmin (monoclonal antibody 33) demonstrates that {alpha}T-catenin is localized in desmin-expressing peritubular myoid cells. In the negative control (neg), only secondary antibody was used.