Fig. 5. Confirmation of T-catenin/ß-catenin interactions by co-immunoprecipitation (IP). (A) IP from HEK-293 cells transfected with a plasmid encoding Myc-tagged T-catenin. In the western blots (left), which serve as controls for efficient transfection, T-catenin was detected by monoclonal antibody 892_24D2S and ß-catenin by a polyclonal antibody. The IP results (right) were obtained either with monoclonal anti-ß-catenin antibody or with monoclonal anti-Myc antibody. SDS-PAGE was followed by western blotting. A mixture of both antibodies was then used to probe this blot. In mock transfected cells, only ß-catenin was detected as expected (data not shown). (B) IP from mouse tissues, performed with polyclonal antibody #952, specific for T-catenin, and a polyclonal antibody specific for ß-catenin (Sigma). After western blotting of total lysates (left) and coimmunoprecipitates (right), T-catenin and ß-catenin were detected by use of the same antibodies.