JCS -- Landsverk et al. 114 (18): 3255 Figure IG1

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Fig. 1. Subcellular distribution of AKAP95 and RII ${alpha}$ in Reh, Reh-RII ${alpha}$ and Reh-RII ${alpha}$ (T54E) cells. (A) Assessment of AKAP95 and RII ${alpha}$ in Reh, Reh-RII ${alpha}$ and Reh-RII ${alpha}$ (T54E) cells. Lysates from interphase and mitotic cells (10⁶ cells per lane) were analyzed by immunoblotting using mAbs against human AKAP95 (mAb47) and human RII ${alpha}$ . (B,C) Immunofluorescence analysis of interphase (B) and mitotic (C) cells using affinity-purified anti-AKAP95 polyclonal antibodies (green) and anti-RII ${alpha}$ mAbs (red). DNA was counterstained with Hoechst 33342 (blue). Bars, 10 µm.