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Fig. 5. Western blot analysis of cytosolic and cytoskeleton fractions prepared from fibroblasts. Control Dupuytren’s fibroblasts (Lane 1) or TGFß-treated cells (lane 2) and lung fibroblasts (Lane 3) were fractionated in cytosolic pool (left panel) and cytoskeleton pool (right panel) and probed for the presence of cytoskeletal proteins by western blot.