Fig. 7. Incorporation of rED-A into cellular FN fibers. Fibroblasts were cultured for 4 days in TGFß and then for 3 hours in the presence of 50 µg/ml of biotinylated rED-A (A,B). (A) Biotinylated rED-A (red) displays a preferential incorporation (yellow) into thick fibers of the ED-A FN (green) network. (B) Vinculin (red) in FAs is partially overlapping (yellow) with rED-A (green) fibers. (C,D) Overlay binding assays: DOC-insoluble matrix prepared from TGFß-treated fibroblasts was overlaid with biotinylated rED-A (C) or r11A12 (D) at 50 µg/ml, 3 hours. (C) rED-A (red) primarily colocalises with thick fibers of FN (green). (D) The recombinant protein r11A12 does not decorate FN fibers (green). Bars, 10 µm.