Fig. 6. The mGCL and LAP2ß proteins regulate the transcriptional activity of the E2F5-DP3 heterodimer. The E2F reporter (0.5 µg) and pCMV-ß-gal (0.5 µg), together with expression vectors for E2F5 (150 ng), DP3 (300 ng), Rb (1 µg), mGCL (1 µg), LAP2ß (1 µg) and LAP2 (1 µg) were transfected into H1299 cells as indicated. The values shown represent the average of duplicate readings and represent the level of luciferase relative to the ß-galactosidase derived from the internal control. The values are presented as activation compared with the mock control.