Fig. 7. Coexpression of munc-18-2 with syn1A does not alter the localization of the t-SNARE in polarized MDCK cells. Cells were cotransfected with syn1A and munc-18-1 (SYN1(M-18-1), A,A', C,C') or munc-18-2 (SYN1 (M-18-2), B,B'). The polarized monolayers were grown as described in Fig. 5. The cells were immunolabeled for syn1A (A,B) or the -subunit of the Na⁺/K⁺ ATPase (C) whereas the apical membrane was stained with concanavalin A (CONA, A',B',C'). Confocal sections in the vertical plane are shown with the apical surface (AP) of the cells at the top.