Fig. 1. Context-dependent induction of CL-1 expression by invasin. RSFs were plated on FN- or invasin-coated dishes. Beads coated with INV497 or fibronectin were added to cells spreading on FN or INV497. CL-1 expression was measured in the supernatant by slot blot 24 hours later. The error bars represent ± s.e.m. of two or more independent experiments. INV497-coated beads produced a large increase in CL-1 secretion, whereas FN-coated beads did not in cells plated on FN. The difference in CL-1 produced in response to FN-coated beads in cells plated on FN, compared to cells plated on INV497 was small, but significant (P=0.046).