Fig. 6. Bead phagocytosis induces TNF- and IL-1 expression, however only IL-1 production is required for bead-induced CL-1 expression. (A) Time course of TNF-, IL-1 and CL-1 production after addition of anti-5 mAb-coated beads. The cytokines and CL-1 were measured in the culture supernatant by slot blot. Error bars represent ± s.d. from one of three independent experiments. (B) CL-1 expression was measured in the supernatant by slot blot 24 hours after the addition of beads coated with anti-5, IL-1 (2 ng/ml) or TNF- (1 µg/ml) in the absence or presence of IL-1 receptor antagonist (400 ng/ml), anti TNF- (8 µg/ml) or anti IL-1 (10 µg/ml). Error bars represent ± s.d. from one of three experiments. *, the effect of anti IL-1 on TNF-induced CL-1 expression was not assayed.