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Fig. 2. RP-A foci on restriction-endonuclease-treated sperm chromatin form with different types of egg extracts. Immunofluorescence with purified rabbit anti-RP-A70 antibodies on intact sperm heads (Sperm) or sperm heads pre-treated with 0.05 units of EcoRI (Sperm+RE) incubated with interphase HSS, interphase LSS or mitotic HSS for 1 hour at 23°C. A cumulative image of 10-12 confocal sections is shown for each sample. Bars, 5 µm.