Fig. 9. Expression of uPA and uPAR is regulated by ERK in MDA-MB-231 cells. MDA-MB-231 cells were treated with PD098059 (10 µM or 50 µM) or with vehicle for 24 hours in serum-free medium. (A) Levels of uPA, cell-associated uPAR and soluble uPAR in CM were determined by immunoblot analysis (representative of three separate experiments). (B) PGAD, uPA and uPAR mRNA levels were analyzed in total RNA isolates.