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Fig. 4. The phosphatases that dephosphorylate p42/p44 MAPKs in the nucleus are neo-synthesized. Quiescent CCL39 fibroblast cells were stimulated for 3 hours with 10% serum (a,d). 30 µM cycloheximide (b,e) or 5 µg/ml actinomycine D (c,f) were added throughout serum stimulation. Indirect immunofluorescence detection was performed either with the polyclonal antibody anti-p42/p44 MAPKs from UBI (green; a,b,c) or with the monoclonal antibody anti-activated p42/p44 MAPKs (red; d,e,f).