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Fig. 2. Glycerol gradient fractionation of protein complexes containing ING1. Extracts prepared from Hs68 fibroblasts and sedimented, as outlined in Materials and Methods, were fractionated from the bottom of tubes. Fraction 15 represents the highest density fraction and fraction 1 represents the least dense fraction that was isolated last from the top of gradients. Samples corresponding to the 15 fractions isolated were electrophoresed through 12.5% gels, transferred to nitrocellulose and probed with (a) {alpha}-ING1 (Cab1-4) or (b) mouse {alpha}-PCNA antibodies and visualized by ECL. Numbers below panel b represent the relative migration of denatured protein standards run on control gradients.