Fig. 1. Generation of LB-TRv transfectants. (A) Schematic map of the CD44 gene. Arrowheads mark the positions of 5'-ORF and 3'-ORF primers (sequence as shown in B) used to isolate the CD44v4-v10 cDNA from mouse keratinocytes. Arrows mark the positions and directions (sense ; anti-sense ) of the primers (for sequence, see Rochman et al. (Rochman et al., 2000) used in the exon-specific RT-PCR analysis of LB cells transfected with isolated CD44v4-v10 cDNA (LB-TRv cells). Hatched bars denote 5' and 3' constant exons (excluding introns), open bars represent the variant exons, the lines connecting the exons depict intron sequences. Pc5 and pv1 to pv10 primers were used for exon-specific PCRs in combination with the 3' primer pc16, for analysis of mouse cDNA. (B) ORF-5' and ORF-3' primers.