Fig. 7. The effect of hyaluronidase and anti-CD44 mAb on local tumor growth and lymph node invasion by LB-TRv lymphoma cells. BALB/c mice were s.c. inoculated with 3x10⁶ green LB-TRv cells (TRv-G). After 6 days, PBS (TRv-G), 20 units heparinase (TRv-G + HEP’ase) or hyaluronidase (TRv-G + HA’ase) of equivalent specific activity was injected i.p. into the mice (A,B). Using the same injection protocol, but in a different experiment, other groups of mice received PBS (TRv-G), isotype-matched control mAb (TRv-G+4D2) or IM7.8.1 anti-CD44 mAb (TRv-G+anti-CD44) (C,D). PBS, enzymes and antibodies, were injected every other day for 12 days (a total of 6 injections). Five mice from each group were killed at each time point). Local tumor size (A,C) and the intensity of lymph node invasion (B,D) were assessed at different time points, as described in Fig. 6. Statistical analysis as indicated in Fig. 6; all groups were compared with the TRv-G group. Note that the intensity of lymph node invasion following herparinase (B) or antibody injection (D) was assayed on day 19 only.