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Fig. 1. Endogenous SRP19 is localized to the nucleus and the nucleolus. (A,B) SRP19 in HeLa cells was detected using indirect immunofluorescence followed by confocal microscopy (see Materials and Methods). Antibodies against fibrillarin and TRAP{alpha} (Görlich et al., 1990) were used as controls for nucleolar and rough endoplasmic reticulum subcellular staining. SRP19 was found to be prominent in the nucleoli and in the nucleoplasm, noticeable especially in the paraformaldehyde-fixed cells. Images were collected using the 63x oil objective of a Leica DM IRB/E microscope and analyzed with Leica TCS NT software. Bar, 10 µm. (C) A western blot showing that the anti-SRP19 antibody at 1:2000 dilution detected only SRP19 (*) in a total HeLa cell lysate.