Fig. 2. Effect of tunicamycin on the labeling and the transport of the D₂ receptors to the plasma membrane. COS-7 cells transfected by the myc-tagged D_2b receptor were treated with tunicamycin for 12 hours at 10 ng/ml (B) and 20 ng/ml (C), and compared with control cells (A). The receptors were visualized with the monoclonal 9E10 antibody, coupled to Texas-Red. Note the massive restriction of the receptor localization close to the nuclear membrane with increasing doses of tunicamycin compared with control cells. Bar, 3 µm.