Fig. 3. Comparison of the distribution of the D_2b receptor revealed by anti epitope-tag antibody and GFP-tagged fusion in transiently transfected COS-7 cells. The labeling exhibited by the D_2b receptor fused to the GFP is seen both intracellularly and at the plasma membrane (A,B) and is very similar to that obtained with anti-D₂ receptor polyclonal antibody, coupled to Texas-Red (C). In sharp contrast, the D_2b receptor-GFP construct (B) provides a visualization significantly different from that obtained with the monoclonal 9E10 antibody, coupled to Texas-Red (D), which revealed mainly intracellular compartments. Confocal microscope images; bars, 1 µm.