Fig. 6. The D₂ receptor isoforms are poorly co-localized with Rab6, a marker of the Golgi complex, in COS-7 and HeLa cells. As analyzed with a confocal microscope, the labeling for the D_2a isoform obtained with the monoclonal P5D4 antibody, coupled to FITC, in HeLa cells (A) and with the monoclonal 9E10 antibody, coupled to FITC, for the D_2b isoform (C), overlap only for a very small part with that obtained with an anti-rab6 polyclonal antibody, coupled to Texas-Red, (B,D). The D_2a receptor transfected in COS-7 cells displays essentially the same pictures (E,F). This receptor labeling is not significantly modified by cell treatment with BFA (10 µg/ml) for 1 hour (G), whereas that of Rab6 spreads over the cytoplasm (H). Bar, 2.5 µm.