Fig. 6. Excitation and emission scans of filipin complexed to boar sperm cells. Sperm cells were labeled with filipin (see Materials and Methods) and suspensions of 10⁶ labeled cells/ml were pipetted in quarts cuvettes and placed in a fluorimeter. The excitation of complexed filipin was detected at an emission wavelength of 480 nm over the range of 275-400 nm (solid line). The emission of complexed filipin was detected at 357 nm excitation (one of the excitation peaks) at a range of 400-600 nm (broken line).