Fig. 9. Morphology scores of the boar samples after a two hour incubation in HBT-Bic supplemented with 0.3% (w/v) BSA. The filipin and M540 response was determined as described in Fig. 8. The sperm samples were also subjected to morphological examination: Sperm cells were diluted, fixed and examined under a phase-contrast microscope. Ejaculates from ten boars were collected (three ejaculates from each boar) and counted in triplicate. The percentage of sperm cells with a normal mature morphology are expressed on the x-axis whereas the percentage of sperm cells with the bicarbonate response is indicated on the y-axis: , cells that stained positively with M540; , cells with filipin response (either labeling patterns B or C; Fig. 7). 200 cells were counted in triplicate and mean values with s.d values (n=10) are expressed for morphology (horizontal error bar) and for M540 or filipin response (vertical error bar).