Fig. 2. mmm1 mutant cells exhibit spherical mitochondria, which are distinct from the aggregated mitochondria in ssc1 mutant cells. Wild-type (MMM1, panels a,b) and temperature-sensitive mmm1 mutant (mmm1-1, panels c,d) cells were grown in YPD at 23°C to an early log phase. Aliquots from the cells grown at 23°C were shifted to 37°C (panels b,d) for 60 minutes. The cells were fixed and prepared for immunofluorescence microscopy with anti-Tom40 antibodies. Bar, 2 µm.