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Fig. 7. The actin cytoskelton is normal in temperature-sensitive mtHsp70 mutant and {Delta}mdj1 cells. Wild-type (a,b), temperature-sensitive mtHsp70 mutant (ssc1-2, panels c,d, ssc1-3, panels e,f ) and {Delta}mdj1 mutant ({Delta}mdj1, panels g,h) cells were grown in YPD at 23°C to an early log phase. Aliquots from the cells grown at 23°C were shifted to 37°C for 60 minutes. The cells were then fixed and the actin cytoskelton was stained with rhodamine-conjugated phalloidin. Bar, 2 µm.