Fig. 6. High resolution confocal images of NPC clustering. BRL cells were incubated with 1 µM staurosporine for 15 hours and subjected to immunostaining using mAb414 and/or anti-lamin B antibodies. DNA was visualized using BoBo-3-iodide. (A) Distribution of nuclear pore proteins (green) and lamin B (red) in 0.3 µm equatorial sections of nuclei in different stages of apoptotic progression. The right panels show enhanced versions of the merged mAb414 and anti-lamin B staining in order to better visualize the weak lamin B staining. Note the interrupted and accumulated pattern of mAb414 immunofluorescence in stage II and III. Bar, 5 µm. (B) Distribution of nuclear pore proteins (green) and chromatin (red) in (0.3 µm) sections of stage II and III nuclei, respectively. Bar, 10 µm. (C) A projection of a stack of optical sections of a nucleus between stage II and III that had been stained with mAb414 antibodies (green) and Bobo-3-iodide (red).