Fig. 6. Double fluorescence analysis of cells stably expressing EGFP-1-448mDia1. For each pair of images, the EGFP fluorescence is shown on the right, TRITC-phalloidin staining (indicative of F-actin) and immunostaining for ezrin and RhoA, respectively, are shown on the left. Note that these ruffles contain the cytoskeletal proteins actin and ezrin, which colocalize with the RBD (as contained in the 1-448mDia fragment) and its ligand RhoA. Bar, 20 µm.