Fig. 7. Supertransfection of 1-448-mDia-expressing cells with dominant negative myc-tagged Rac1 (N17Rac1). (A,A’) Double fluorescence analysis with antibody against the myc-tag (A) and EGFP fluorescence (A’), to detect the N17Rac1-expressing cells among the 1-448mDia-expressing clone 1A cells (arrowheads). Bar, 20 µm. (B) Quantitation of ruffling activity of clone 1A cells coexpressing N17Rac1, identified from images as shown in (A). Note that N17Rac1 is effectively suppressing ruffling. At least 300 cells were counted, in four independent experiments.