Fig. 8. Directed locomotion of CHO clones expressing EGFP-1-448mDia1 (clones, 1A and 1B) or EGFP only (control). Directed locomotory activity was recorded under phase-contrast as the velocity to close a wound set in the confluent culture, at the times indicated. Cells were kept and monitored either without (A) or with (B) colcemid in the medium. Note that wound closure is considerably faster in the control culture than in both RBD-expressing clones. Colcemid enhances this effect, possibly by inhibiting cell proliferation. Bar, 1 mm.