Fig. 4. Synapsin I localizes to the Golgi area of NRK cells. Cells were double immunostained for synapsin I (SynI) (A,a, B,b) and mannosidase II (mann II) (A’,a’) or TGN38 (B’,b’). Boxed regions in A, A’,B,B’ are shown enlarged in a,a’,b,b’, respectively. Asterisks mark cell nuclei. Bar, 10 µm. (C) Confocal sections acquired at different levels of the Golgi of a NRK cell double-stained for synapsin I (green) and TGN38 (red). Consecutive sections are 0.5 µm apart. Bar, 5 µm.