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Fig. 6. Colocalization studies between synapsin I and different markers in NRK cells. NRK cells were double stained with SNT1 (A,a,B,b,C,c,D,d,E,e) and antibodies against {gamma} adaptin (A’,a’); the transferrin receptor (Tf R) (B’,b’); ßCOP (C’,c’); syntaxin 6 (D’,d’); and SCAMP4 (E’,e’). Boxed regions in A,A’,B,B’,C,C’,D,D’,E,E’ are shown enlarged in a,a’,b,b’,c,c’,d,d’,e,e’, respectively. Synapsin I staining localizes to the juxtanuclear region of the cells as well as staining for the other markers. However, in all cases, the synapsin I compartment does not appear to be stained by the second antibody. Asterisks mark cell nuclei. Bar, 10 µm.