Fig. 7. Synapsin I and myosin II colocalize at the Golgi region in NRK and WIF-B cells. Methanol-fixed NRK (A,A’,a,a’) and WIF-B (B,B’,b,b’) cells were double-stained for synapsin I (A,a,B,B) and myosin II (A’,a’,B’,b’). Both antigens localize to the same juxtanuclear structures in these cells. Boxed regions in A,A’,B,B’ are shown enlarged in a,a’,b,b’, respectively. Non-Golgi staining of myosin II antibody is due to labeling of stress fibers. Asterisks mark cell nuclei. Stars mark an apical cyst enclosed by two polarized WIF-B cells. Bar, 10 µm.