Fig. 6. Electron micrographs of mutant dynamin-expressing HeLa K44A (A) and induced BHK antisense CHC cells (B,C) showing internalized CTB-HRP conjugate. In A, HeLa K44A cells were incubated with CTB-HRP conjugate for 30 minutes at 37°C before processing for EM. The CTB-HRP labeling was seen in an endosome (En) and surrounding tubulo-vesicular structures. In B,C, BHK antisense CHC cells were similarly incubated with CTB-HRP conjugate for 30 minutes at 37°C. Labeling was seen in an endosome (En) and surrounding tubulo-vesicular structures (B) as well as in a cistern (arrow) of a Golgi complex (Go) (C). Bars, 200 nm.