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Fig. 2. Nek2 and Cdk2 induce centrosome splitting through independent pathways. U2OS cells were co-transfected with different combinations of wild-type Cdk2 and Nek2, or catalytically inactive Cdk2 (D145N) and Nek2 (K37R), together with cyclins A or E as indicated. Centrosome splitting was then analyzed as described in Fig. 1 legend.