JCS -- Raucher and Sheetz 114 (20): 3759 Figure IG3

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Fig. 3. PLC ${delta}$ PH domain-GFP relocalization in chlorpromazine. (a) Confocal fluorescent image of membrane localization of PLC ${delta}$ PH domain-GFP fusion protein in transfected NIH-3T3 cells. The fluorescent intensities along the white lines were plotted as line intensity histograms, shown in the right panels. Calculation of I_pm/I_cyt was used to quantify the extent of membrane localization. (b) Localization of PLC ${delta}$ PH domain-GFP (left panel) and fluorescence intensity changes (right panel) after incubation with 30 µM chlorpromazine.