Fig. 4. Time course of PIP₂ relocalization and membrane tension in the presence of local anesthetics. (a) Fluorescence ratios calculated from line intensity plots (see legend to Fig. 3) were plotted against time. Chlorpromazine or met-chlorpromazine was added at 0 seconds, and images were taken at 5 minute intervals. (b) Tether force was measured as described in Fig. 1 and plotted against time. Chlorpromazine or met-chlorpromazine was added at 0 seconds, and measurements were made at 5 minute intervals. CPZ, chlorpromazine; M-CPZ, met-chlorpromazine.