Fig. 6. Calcium response induced by (a) chlorpromazine (CPZ) alone and (b) chlorpromazine combined with the PLC inhibitor U73122. Fluorescence images were acquired using confocal time-lapse imaging of NIH-3T3 fibroblasts loaded with the Ca(2+)-sensitive dye fluo-3. Fluorescence intensity of each image was quantified as the sum of all pixel intensities.