JCS -- D’Amours et al. 114 (20): 3771 Figure IG2

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Fig. 2. Affinity purification of the apoptotic fragments of PARP-1. Bovine PARP-1 (purified to homogeneity) was cleaved with caspase 3 and subjected to ssDNA-cellulose affinity chromatography as described in Material and Methods. Silver-stained SDS-polyacrylamide gel (Desnoyers et al., 1995) showing the apoptotic fragments of PARP-1 obtained in late ( $~$ 500 mM NaCl; 24 kDa) and early (89 kDa) fractions of the chromatography procedure.