Fig. 2. Cellular localization of Pin1p. (A) Schematic diagram of how the pin1⁺-GFP fusion chimera integrated into the chromosome. (B) The Pin1p-GFP expressing strain (Sp35) was grown to late log phase. Cells were fixed in 3.7% formaldehyde for 30 minutes followed by DAPI staining for 10 minutes in the dark. Cells were viewed with a Leitz Laborlux microscope and images were acquired by Adobe PhotoShop software using the SPOT-2 CCD digital camera.