Fig. 4. Deletion analysis of ESS1 and pin1⁺. (A) Wild-type diploid S. cerevisiae cells deleted for one copy of ESS1 were subjected to sporulation followed by tetrad dissection. Spores were grown on YEPD plates at 17°C for 9 days (left panel), or at 30°C for 4 days (middle panel), or at 37°C for 6 days (right panel). (B) A wild-type, two independent S. pombe pin1 strains and a rad3 strain were grown in YES medium overnight and then plated on YES plates followed by UV irradiation. Plates were cultured at 32°C with a foil cover for 1 day and without a foil cover for the subsequent 3 days. Colony numbers were calculated and survival rates were determined based on control plates, which were not irradiated. The data in this figure represent the average of three independent experiments with a standard deviation of less than 7%.