Fig. 3. FIGQY phosphorylation is concentrated in paranodal regions of adult sciatic nerve. Sections of adult rat sciatic nerve were labeled with either the phospho-FIGQY polyclonal antibody (A,D,G) or a neurofascin-specific polyclonal antibody (J). Nodes of Ranvier were visualized with the monoclonal antibody against ankyrin G (B,E,H,K). Composite images (C,F,I) demonstrate the minimal overlap between nodal ankyrin G (green) and paranodal phospho-FIGQY (red). The neurofascin-specific polyclonal antibody recognizes both nodes and paranodes (J-L). The phospho-FIGQY polyclonal antibody in G was cytosol cleared, demonstrating no difference in the pattern of immunofluorescence with cytosol clearing (see Materials and Methods for details). White arrowheads indicate the location of nodes of Ranvier. Scale bars: 5 µm in A-I; 2.5 µm in J-L.