Fig. 5. FIGQY phosphorylated neuroglian is present at the developing neuromuscular junction and at sites of muscle-tendon attachment in Drosophila embryos. (A) The developing neuromuscular junction of Drosophila embryos is shown as marked by Fas II labeling (white arrowhead). FIGQY-phosphorylated neuroglian is present at the neuromuscular junction (B, C for composite). (D-F) Embryos were labeled with an anti neuroglian antibody (D) and the phospho-FIGQY polyclonal antibody (E). A composite image is shown in F, with the developing neuromuscular junction marked by a white arrowhead. The phospho-FIGQY polyclonal antibody also labels muscle-tendon attachment sites (B-I, yellow arrowheads) as indicated by overlap with PS2 (G) (yellow arrowheads). Composite image shown in (L). Neuroglian is also present at these sites (D-F, yellow arrowheads) although it is not dramatically enriched. Scale bars: 10 µm. A, axon of presynaptic nerve cell; M, muscle cell.