Fig. 2. RT-PCR analysis of alternative splicing events in (A) the N- and (B,C) C-terminal region of mARVCF mRNA. The mRNA used for RT-PCR was isolated from differentiating mouse myoblasts (i28), total mouse heart (heart) and CMT-cells derived from mouse colon carcinoma (CMT). (A) Agarose gel electrophoresis of RT-PCR products amplified with a sense primer in the 5'UTR and an antisense primer within exon 6 amplifying both N-terminal isoforms at once. (B) Agarose gel electrophoresis of RT-PCR products amplified with a sense primer in exon 15 and an antisense primer in exon 20 amplifying all four C-terminal isoforms at once. (C) Agarose gel electrophoresis of RT-PCR products amplified with one sense primer in exon 15 and two antisense primer in exon B and exon 19. (D) Agarose gel electrophoresis of RT-PCR products amplified with (1) oligonucleotide primers specific for the armadillo repeat region of ARVCF which is not affected by alternative splicing (upper band) and (2) oligonucleotide primers specific for the housekeeping gene BIP (binding protein) as a standard (lower band). The mRNA used for RT-PCR was isolated from the cell lines indicated above. (FL, full length).