Fig. 4. Cellular localisation of mARVCF splice variants. Mouse myoblasts i28 (A), MCF7 cells (B), RT112 (C), EJ28 (D), HeLa cells (E) and COS-7 cells (F) were transfected with the eight potential isoforms as EGFP-fusion constructs and expression visualised by fluorescence microscopy. The localisation of the eight potential isoforms was seen at the cell membrane in all cell lines tested with the exception of EJ28 cells. Here, as a representative result for the different isoforms, the localisation of EGFP-FL-3/7 is shown. As controls, each cell line was transfected with the empty EGFP-plasmid (A-co to F-co). In i28 cells, the endogenous M-cadherin was detected with M-cadherin antibodies (Ai), in MCF7 (Bi) and RT112 (Ci) cells endogenous E-cadherin was detected with the E-cadherin antibody and N-cadherin was detected with the anti A-CAM antibody in EJ28 (Di) and HeLa cells (Ei). In COS-7 cells the anti-pan-cadherin antibody was used to detect endogenous cadherin(s) (Fi). (Am-Fm) merged images of (A-F) and (Ai-Fi). Bars, 10 µm.