
Fig. 5. GST binding assay. In vitro translated 35S-methionine-labeled mARVCF splice variants FL-C11 (lane 1) and FL-3/7 (lane 2) were incubated with the cytoplasmic domains of M- (lanes 4,8), E- (lanes 5,9) or N-cadherin (lanes 6,10) as GST-fusion proteins. As controls, in vitro translated 35S-methionine-labeled mARVCF splice variants FL-C11 (lane 3) and FL-3/7 (lane 7) were incubated with GST alone. (GST, glutathione-S-transferase).